Which of the following is not a scaffold fabrication technique?

Centrifugation is not typically considered a scaffold fabrication technique in tissue engineering. The primary purpose of centrifugation is to separate components based on their density and is commonly used in various laboratory processes, such as isolating cells or precipitating proteins.

In contrast, gas foaming, rapid prototyping, and electrospinning are all methods specifically designed to create scaffolds that provide structural support for tissue growth.

Gas foaming involves incorporating gas into a polymer matrix to create pores, which are essential for nutrient transport and cell proliferation. Rapid prototyping utilizes computer-aided design and manufacturing to create scaffolds with specific shapes and structures, often allowing for customization. Electrospinning involves using electrical forces to produce fine fibers from a polymer solution, creating a fibrous scaffold that mimics the extracellular matrix.

Thus, the focus on generating actual scaffold structures distinguishes the other techniques from centrifugation, which does not contribute to the physical creation of scaffolds but rather serves a different role in biological material processing.