Which of the following are effects of equiaxial strain applied using EQUicycler on cell viability?

The position that EQUicycler maintains and promotes MC3T3 cell viability over extended loading-culture conditions is grounded in the fundamental principles of mechanobiology and tissue engineering. The application of equiaxial strain, like that which is produced by the EQUicycler system, activates cellular responses that can enhance cell survival and proliferation under mechanical stress.

When cells are subjected to mechanical loading, they often adapt by triggering biochemical pathways that promote cell health, such as increased production of growth factors and extracellular matrix components. This is particularly important for bone cells like MC3T3, which are osteoblast-like and benefit from mechanical stimuli for their differentiation and functionality.

Extended periods of mechanical loading, when balanced correctly, can lead to an environment that supports not just cell viability but also the maintenance of their differentiated state and activity. This adaptability is key in tissue engineering, where the goal is to create scaffolds or environments that mimic natural physiological conditions, facilitating the growth and maintenance of tissue-like structures.

In this context, the other options, while they may reflect phenomena observed in cell behavior under strain, do not directly pertain to the aspect of cell viability in the same conclusive manner as the selected statement. Aligning cells and adopting elongated morphologies are