What was the effect of equiaxial mechanical strain on secreted collagen levels?

The correct answer indicates that all loaded samples showed higher secreted collagen levels in response to equiaxial mechanical strain. This finding aligns with the principles of mechanobiology, where mechanical stimuli can positively influence cellular behaviors, particularly in cells associated with tissue repair and regeneration, such as fibroblasts and chondrocytes.

When these cells are subjected to equiaxial mechanical strain, they often respond by increasing the synthesis of extracellular matrix components, including collagen. Collagen is crucial for the structural integrity and function of various tissues, and its elevated levels can enhance tissue quality and resilience. This process exemplifies the concept of mechanotransduction, where mechanical signals are converted into biochemical responses. Therefore, mechanical loading stimulates the synthesis of collagen to adapt to the increased demand or stress, ultimately aiding in tissue remodeling and repair.

The other options, while relevant to tissue engineering and cellular responses, do not reflect the specific outcome of equiaxial mechanical strain on collagen secretion as described by the correct option.