What strain percentage and frequency will be used to mechanically stimulate biphasic scaffolds for annulus fibrosis repair?

The selected answer indicates using a strain percentage of 6% with a frequency of 1 Hz for mechanically stimulating biphasic scaffolds intended for annulus fibrosus repair. This combination is significant because it reflects the physiological conditions that the annulus fibrosis would normally encounter in vivo, thus promoting appropriate cellular responses and matrix production.

Mechanical stimulation, particularly strain, is crucial in tissue engineering as it can enhance the mechanical properties and biological functions of scaffold materials. The optimal range often considered for this type of tissue, particularly in the context of mimicking natural lumbar conditions, tends to favor lower strain percentages (like 6%) which can initiate metabolic activities without overloading the cells, risking injury or adverse outcomes.

A frequency of 1 Hz aligns well with the physiological loading conditions experienced by the intervertebral disc, particularly during normal body movements. This frequency allows the cells in the scaffold to respond effectively to mechanical cues necessary for repair and regeneration while maintaining the integrity of the tissue structure.

This rationale underlies the choice of these parameters in repair strategies, emphasizing the importance of simulating the natural environment of the tissue to achieve successful outcomes in tissue engineering initiatives.