What is a limitation of Direct Fabrication?

Direct Fabrication, particularly in the context of tissue engineering, often relies on techniques such as 3D printing or additive manufacturing. One notable limitation of this method is that it can expose materials to harsh heat and chemical environments during the fabrication process. This can affect the integrity and functional properties of the materials used to create scaffolds.

For example, certain polymers may degrade or lose their bioactivity when subjected to high temperatures, which can impact their ability to support cell adhesion, growth, and function. Additionally, the use of certain solvents or chemicals during the fabrication process can potentially harm sensitive bioactive molecules that may be intended for later delivery or integration into the tissue constructs.

While other options present potential concerns in different contexts, the issue of harsh environmental conditions is particularly pronounced in Direct Fabrication, making it a significant limitation in the pursuit of creating effective scaffolds for tissue engineering applications.