What are the two deposition methods of direct cell writing?

In the context of direct cell writing, which is a technique used in tissue engineering for the precise placement of cells or biomaterials, the two deposition methods identified are drop-on-demand and continuing.

Drop-on-demand refers to a method where droplets of cell suspension or bioink are ejected from a nozzle only when required, allowing for greater control over the placement and arrangement of the cells. This precise control is crucial for creating complex tissue structures and ensures that the cells are deposited exactly where needed without excess material.

Continuing deposition, on the other hand, involves a continuous flow of bioink or cell suspension from the nozzle, leading to a steady stream of material being laid down. This technique is beneficial for creating longer, uninterrupted patterns or structures, allowing for rapid filling of areas and is particularly useful when creating scaffolds or larger constructs.

These two methods encapsulate the fundamental approaches to direct cell writing, each offering distinct advantages for specific applications in tissue engineering.