True or False: Scleraxis and Tenascin were downregulated for the 6% and 12% strain groups loaded at both 0.1 Hz and 1 Hz frequencies.

The assertion focuses on the regulation of Scleraxis and Tenascin in response to mechanical strain at different frequencies. To determine the validity of the statement, it's essential to understand the roles of Scleraxis and Tenascin in tissue engineering, particularly in the context of mechanical loading.

Scleraxis is a transcription factor linked to the regulation of tendon development and is generally responsive to mechanical loading. When tendon cells are subjected to strain, Scleraxis is typically upregulated to promote matrix synthesis and adaptation to load. Similarly, Tenascin is an extracellular matrix protein that plays a role in cellular signaling during tissue remodeling and repair.

The statement indicates that both Scleraxis and Tenascin were downregulated in the 6% and 12% strain groups at frequencies of 0.1 Hz and 1 Hz. However, it is often the case that these factors respond positively to mechanical stimuli, indicating their role in adaptive processes. If Scleraxis and Tenascin were indeed downregulated, it would imply an inhibition of the cellular responses necessary for tissue adaptation to strain.

Based on the information provided, the correct answer is that the statement is false. This suggests that either Scleraxis and Tenascin were not downregulated under the