For cell proliferation, do cells grow to form a monolayer on the surface and stop growing once a critical surface density is achieved?

In the context of cell proliferation, when cells are cultured in a monolayer, they indeed grow until they reach a certain surface density, at which point their proliferation typically ceases. This phenomenon is often referred to as contact inhibition, a regulatory mechanism that ensures cells stop dividing when they are in close proximity to one another, thus preventing overcrowding.

Once the critical surface density is reached, cells signal each other through various mechanisms such as cell-cell adhesion molecules and growth factor signaling, leading to a halt in further division. This behavior is essential for maintaining tissue architecture and function, as uncontrolled proliferation can lead to conditions such as tumors. Therefore, the statement aligns well with the principles of cell growth in tissue engineering and cell culture practices, indicating that the correct answer is true.