Applying homogenous equiaxial strain to tissue-engineered scaffolds encapsulated with human ASCs can lead to what cellular effect?

When applying homogenous equiaxial strain to tissue-engineered scaffolds that contain human adipose-derived stem cells (ASCs), the mechanical environment plays a crucial role in determining the cell behavior and differentiation pathway. The correct response indicates that such mechanical stimulation can modulate the cells towards the annulus fibrosus lineage, which is significant in the context of tissue engineering, especially for applications like intervertebral disc regeneration.

Cells are sensitive to mechanical inputs, and the application of strain can influence gene expression, cellular behavior, and ultimately phenotype. The annulus fibrosus is the tough outer layer of intervertebral discs, and its formation is crucial for creating a tissue that can withstand compressive forces. By applying a homogenous equiaxial strain, the environment mimics the physiological loading conditions that cells would experience in vivo, prompting ASCs to differentiate along the appropriate lineage and contribute to the development of fibrocartilaginous tissue.

This differentiation is essential for the repair or replacement of damaged annulus fibrosus tissue, making the modulation toward this specific lineage a relevant and desirable outcome in tissue engineering applications. Thus, the application of mechanical strain serves as a strategic approach to guide the differentiation of ASCs, enhancing their potential use in